Genetic separability of the chorismate mutase and prephenate dehydrogenase components of the Escherichia coli tyrA gene product.
نویسندگان
چکیده
Fragments of the tyrA gene of Escherichia coli, when suitably engineered, can express either the chorismate mutase activity or the prephenate dehydrogenase activity without the other.
منابع مشابه
Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli: generation and characterization of tyrosine-insensitive mutants.
In order to get insights into the feedback regulation by tyrosine of the Escherichia coli chorismate mutase/prephenate dehydrogenase (CM/PDH), which is encoded by the tyrA gene, feedback-inhibition-resistant (fbr) mutants were generated by error-prone PCR. The tyrA(fbr) mutants were selected by virtue of their resistance toward m-fluoro-D,L-tyrosine, and seven representatives were characterized...
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عنوان ژورنال:
- Journal of bacteriology
دوره 169 10 شماره
صفحات -
تاریخ انتشار 1987